8:15 am Virtual Coffee & Online Registration

UNDERSTANDING THE TUMOR MICROENVIRONMENT & COMBINATION THERAPIES

8:45 am Chair’s Opening Remarks

9:00 am Modulation Of The Tumor Micro-Environment By Oncos-102 – Experience From Several Types Of Cancer

Synopsis

• ONCOS-102 acts synergistically to reduce tumor volume with the chemotherapy combination of pemetrexed and cisplatin (Pem/Cis), which is the current standard of care in malignant pleural mesothelioma
• Demonstration that ONCOS-102 induces CD8+ T-cells specific to the tumor associated antigen (TAA) mesothelin, which is typically overexpressed in mesothelioma, as well as many other forms of cancer

9:30 am Resurrecting and Supporting the Immune Response with Combination Therapy to Cure Pancreatic Ductal Adenocarcinoma

Synopsis

• Utilizing therapeutic interventions to create an internal vaccine.
• Rational utilization of PD-1 inhibitors in the treatment of PDAC.
• Complementing the immune response against cancer with EGFR/KRAS combination therapy

10:00 am Neoantigen Vaccination for Glioblastoma: Does Timing of Anti-PD-1 Matter?

  • David Reardon Clinical Director, Center for Neuro- Oncology, Dana-Faber Cancer Institute

Synopsis

• Better understand the rationale of combining neoantigen vaccination with anti-PD-1 therapy;
• Gain awareness of increasing preclinical data implicating how the timing of anti-PD-1 therapy may impact on anti-tumor immune responses generated by neoantigen vaccination;
• Appreciate the challenges of assessing the impact of anti-PD-1 timing on anti-tumor immune responses generated by neoantigen vaccination in a prospective clinical trial.

10:30 am Morning Break & Speed Networking

11:00 am Chromatographic tools for optimization of IVT reaction and improving mRNA purification process

  • Rok Sekirnik Head of pDNA/mRNA Process Development, BIA Separations, a Sartorius Company

Synopsis

• The recently demonstrated efficacy of mRNA-based Covid-19 vaccines has shown the promise of this therapeutic format, but also highlighted the need for higher efficiency of mRNA production to meet enormous needs for global vaccine supply.
• Typical mRNA production process involves three key steps: 1) plasmid DNA (pDNA) production in supercoiled (sc) isoform, linearization and purification, 2) in-vitro transcription (IVT) reaction and 3) mRNA purification.
• Here we present a chromatographic toolbox for integrated mRNA production from pDNA to mRNA purification, including in-process analytics.

11:30 am Understanding Mechanisms Of Immune Resistance In Nsclc Through Analysis Of The T Cell Repertoire

  • Alexandre Reuben Assistant Professor, The University off Texas MD Anderson Cancer Center

Synopsis

• Substantial intratumor genomic and T cell heterogeneity (ITH) in earlystage NSCLC
• High frequency of bystander T cells are present in lung tumors
• Patients with more ITH and/or bystander T cells experience worse outcome
• Lung TILs recognize viral antigens
• Bystander TILs exhibit an unfavorable profile for anti-tumor responses
• Animal models allow the analysis of these phenomena and therapeutic strategies to overcome them
• Analysis of these mechanisms in patients treated with immunotherapy underway

12:00 pm Altering the Human Prostate Tumor Immune Microenvironment Through Inhibition of Ibrutinib

  • Russell Pachynski Assistant professor, Division of Oncology, Washington University School of Medicine

Synopsis

• This is the first clinical trial of the BTK inhibitor ibrutinib in prostate cancer
• Ibrutinib may alter favorably alter the prostate tumor microenvironment by reducing pro-tumor B cells and increasing anti-tumor T cells
• Inhibition of BTK may also limit tumor cell-intrinsic growth
• Prostate tissue and peripheral correlative studies will be performed to evaluate the further use of ibrutinib in prostate cancer

12:30 pm Next Generation Cancer Vaccine-Adjuvant Combinations

  • Mark Findeis Executive Director, Research Biochemistry, Agenus Bio

Synopsis

• Heat shock protein as neoantigen peptide chaperone
• High-yield synthesis of neoantigen peptides
• New adjuvant manufacturing and formulations to combine with vaccines

1:00 pm Lunch Break & Virtual Networking

CANCER VACCINE CASE STUDIES: mRNA, DNA, VIRAL VECTOR, PEPTIDE & DENDRITIC CELL

1:45 pm A Novel Heterologous Prime Boost Vaccine System To Drive Tumor Specific T Cell Responses For Cancer Immunotherapy

Synopsis

• Development of a potent heterologous prime/boost immunization approach to deliver predicted TSNAs to patients, which is comprised of a replication incompetent chimpanzee adenoviral vector (ChAdV) for the prime vaccination and a self-replicating, synthetic viral vector (srRNA) for repeated boost vaccinations
• Induction of high titer, polyfunctional and durable T-cell responses against non-self antigens
• Clinical trial updates will be highlighted

2:15 pm Optimizing the Efficacy of mRNA-based Cancer Vaccines to Generate Potent and Long-lasting CD8+ Responses

Synopsis

• Learning from preclinical models to guide and optimize mRNA-based vaccine platforms
• Deciding the best combination therapy to use with mRNA
• Determining anti-tumor T cell efficacy and future directions

2:45 pm Targeting Unique Neoantigens From Individual Patient Tumors To Develop Novel Treatment For Cancer

Synopsis

• Optimized DNA plasmid containing encoded neoantigens, used in combination with IL-2 to boost T cell response
• Electroporation-based delivery system for enhanced and efficient plasmid uptake to allow optimal antigen production
• Combination therapy shows robust functional antigen specific CD4+ and CD8+ killer T cells

3:15 pm Afternoon Break & Speed Networking

3:45 pm New class of Antigen-specific Cancer Active Immunotherapies based on an off-the-shelf Antigen Presenting Cell line

Synopsis

• PDC*line is a new potent and scalable therapeutic cancer vaccines based on a proprietary allogeneic cell line of Plasmacytoid Dendritic Cells
• PDC*line is much more potent to prime and boost antitumor antigen, including neoantigens, specific cytotoxic T-cells than conventional vaccines and improves the response to checkpoint inhibitors
• The technology can be applied for any cancer

4:15 pm VB10.NEO: Development of a Personalized DNA-based Vaccine to Treat a Range of Cancers

Synopsis

• Industry collaboration to generate individualized cancer treatments intended for use as a therapeutic vaccination in patient with locally advanced or metastatic tumors
• Clinical data to show a clear link between selection of high-quality neoepitopes and generation of strong neoepitope-specific CD8+ T cell responses

4:45 pm Viral vectored vaccines targeting many neoantigens elicit effective anti-tumor T cell immunity and response in combination with anti-PD1

Synopsis

• Nouscom proprietary engineered viral vectors encoding large number of tumor neoantigens elicit strong immune response and effectively synergize with checkpoint inhibitors (CPI) to eradicate large tumors in preclinical models
• Effectiveness of vaccination is CD8 mediated and correlates with expansion and diversification of the intratumoral T cell repertoire
• Clinical development in indications with high Tumor Mutational Burden responsive to CPI, with an off-the-shelf vaccine targeting 209 shared frameshift mutations for treatment of MSI tumors and a personalized vaccine targeting up to 60 neoantignes for treatment of NSCLC and melanoma: preliminary clinical evidence

5:15 pm Chair’s Closing Remarks

5:30 pm End of Day One